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Creating effective CMV virus controls for shell vial assay
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HD-NZ
medicine forum beginner


Joined: 19 Jul 2006
Posts: 1

PostPosted: Wed Jul 19, 2006 8:40 am    Post subject: Creating effective CMV virus controls for shell vial assay Reply with quote

Does any one have a procedure on how to propagate and freeze CMV for
use as a positive shell vial control? Some specific questions:
1) Is it better to aliquot the controls so that they are one-time use?

2) Is freezing the specimen better vs. propagating virus in tissue
culture first - then freezing?
3) Is a 50% Sorbitol combined equally with Hanks Balanced Salt a good
medium to freeze CMV?

Thank you!
H
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