Anticancer properties of chlorogenic acid

ironjustice@aol.com · medicine forum Guru Joined: 28 Apr 2005 Posts: 1522

Cancer Cell Int. 2006 Mar 27;6:7. Related Articles, Links

The chemopreventive properties of chlorogenic acid reveal a potential
new role for the microsomal glucose-6-phosphate translocase in brain
tumor progression.

Belkaid A, Currie JC, Desgagnes J, Annabi B.

Laboratoire d'Oncologie Moleculaire, Departement de Chimie, Centre
BIOMED, Universite du Quebec a Montreal, Montreal, Quebec, Canada.
annabi.borhane@uqam.ca.

ABSTRACT : BACKGROUND : Chlorogenic acid (CHL), the most potent
functional inhibitor of the microsomal glucose-6-phosphate translocase
(G6PT), is thought to possess cancer chemopreventive properties. It is
not known, however, whether any G6PT functions are involved in
tumorigenesis. We investigated the effects of CHL and the potential
role of G6PT in regulating the invasive phenotype of brain
tumor-derived glioma cells. RESULTS : RT-PCR was used to show that,
among the adult and pediatric brain tumor-derived cells tested, U-87
glioma cells expressed the highest levels of G6PT mRNA. U-87 cells
lacked the microsomal catalytic subunit glucose-6-phosphatase
(G6Pase)-alpha but expressed G6Pase-beta which, when coupled to G6PT,
allows G6P hydrolysis into glucose to occur in non-glyconeogenic
tissues such as brain. CHL inhibited U-87 cell migration and matrix
metalloproteinase (MMP)-2 secretion, two prerequisites for tumor cell
invasion. Moreover, CHL also inhibited cell migration induced by
sphingosine-1-phosphate (S1P), a potent mitogen for glioblastoma
multiform cells, as well as the rapid, S1P-induced extracellular
signal-regulated protein kinase phosphorylation potentially mediated
through intracellular calcium mobilization, suggesting that G6PT may
also perform crucial functions in regulating intracellular signalling.
Overexpression of the recombinant G6PT protein induced U-87 glioma cell
migration that was, in turn, antagonized by CHL. MMP-2 secretion was
also inhibited by the adenosine triphosphate (ATP)-depleting agents
2-deoxyglucose and 5-thioglucose, a mechanism that may inhibit
ATP-mediated calcium sequestration by G6PT. CONCLUSION : We illustrate
a new G6PT function in glioma cells that could regulate the
intracellular signalling and invasive phenotype of brain tumor cells,
and that can be targeted by the anticancer properties of CHL.

PMID: 16566826 [PubMed - in process]

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Iron Chelation by Chlorogenic Acid as a Natural Antioxidant

Yasuhisa KONO1), Sakiko KASHINE1), Takushi YONEYAMA1), Yuji SAKAMOTO1),
Yoshihisa MATSUI1) and Hitoshi SHIBATA1)

1) Department of Life Science and Biotechnology Faculty of Life and
Environmental Science Shimane University

(Received April 28, 1997)

Chlorogenic acid, a dietary antioxidant, effectively inhibited the
iron-induced lipid peroxidation of bovine liver microsomes in a
concentration-dependent manner. In the Fenton-type reaction,
chlorogenic acid inhibited the production of the hydroxyl radical by
iron-EDTA or iron-ADP, while iron plus chlorogenic acid did not
generate the hydroxyl radical. The formation of an iron complex with
chlorogenic acid was demonstrated by UV/vis absorbance spectroscopic,
ESR and 1H-NMR studies. The ferric complex with chlorogenic acid was in
the ferric high-spin state near rhombicity, and had no radical
scavenging activity. The results indicate that chlorogenic acid
prevented the formation of the hydroxyl radical by forming a chelate
with iron whose complex cannot catalyze the Fenton-type reaction.

Key words: antioxidant; free radical; chelation; chlorogenic acid; iron

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