FAQFAQ   SearchSearch   MemberlistMemberlist   UsergroupsUsergroups 
 ProfileProfile   PreferencesPreferences   Log in to check your private messagesLog in to check your private messages   Log inLog in 
Forum index » diseases » lyme
Extremely important for clinicians - limitations of tests for Lyme disease
Post new topic   Reply to topic Page 1 of 1 [1 Post] View previous topic :: View next topic
Author Message
georgia
medicine forum Guru


Joined: 06 May 2005
Posts: 505

PostPosted: Sun May 07, 2006 7:44 pm    Post subject: Extremely important for clinicians - limitations of tests for Lyme disease Reply with quote

Int J Med Microbiol. 2002 Jun;291 Suppl 33:120-4. Related Articles,
Links

Evidence of a Lyme borreliosis infection from the viewpoint of
laboratory
medicine.

Lange R, Seyyedi S.

Hospital Dienstleistung + Beratung GmbH, Department of laboratory
medicine,
Bernau, Germany. rlange@libb.de

More than 20 years after the first description of the pathogen Borrelia
burgdorferi the diagnosis of Lyme borreliosis is based primarily on
clinical
criteria. Multiple differential diagnoses have to be considered and
laboratory confirmation based on various test procedures is required
until
now. For screening assays the immunofluorescence assay (IFA), enzyme
linked
immunoassay (EIA), or indirect hemagglutination assay (IHA) are
frequently
in use. During the last ten years a stepwise diagnostic procedure in
USA and
Europe has been recommended. The use of a serological screening assay
as the
first diagnostic step, followed by a confirmatory assay only in the
case of
a positive or borderline result of the screening assay is now "state of
the
art". The most frequently used method is a sensitive enzyme linked
immunoassay followed by a sensitive and specific immunoblot technique.
However, the quality of these indirect serological tests and, mbst
importantly, the interpretation criteria of the commercial tests vary
dramatically. The choice of Borrelia strains, antigen preparation,
production conditions and test procedures vary widely. The immunoblot,
used
as a confirmatory assay, must meet the highest quality standards but in
practice under routine laboratory conditions, these levels are often
not
reached. The major reason for this "shortcoming" is that Lyme
borreliosis is
not a sexually transmitted disease and no official approval has been
required by the commercial suppliers so far. Another increasingly
important
aspect is the introduction of molecular biological assays into
laboratory
routines. Numerous authors published many articles about the usefulness
of
polymerase chain reaction as a diagnostic tool for the detection of
borreliae in human specimens. However, a routine method has not yet
been
established. Furthermore, no standardized method for DNA isolation from
different human specimens has been developed so far. For this reason
PCR is
not accepted as a routine method and the application should be
restricted to
a few laboratories with experience in the field of Lyme borreliosis. In
summary, it is important to inform clinicians about the limitations of
the
tests used and it would be very helpful to stage a European or
worldwide
conference to establish accepted rules for the diagnosis of Lyme
borreliosis.

PMID: 12141736 [PubMed - indexed for MEDLINE]
Back to top
Google

Back to top
Display posts from previous:   
Post new topic   Reply to topic Page 1 of 1 [1 Post] View previous topic :: View next topic
The time now is Fri Jul 20, 2018 1:19 am | All times are GMT
Forum index » diseases » lyme
Jump to:  

Similar Topics
Topic Author Forum Replies Last Post
No new posts Battling Lyme disease mark_denton2006@yahoo.com lyme 0 Tue Jul 18, 2006 7:46 pm
No new posts Schumer addresses Lyme disease mark_denton2006@yahoo.com lyme 0 Tue Jul 18, 2006 7:43 pm
No new posts Kelly met with CDC on Lyme disease mark_denton2006@yahoo.com lyme 0 Tue Jul 18, 2006 7:42 pm
No new posts Rep. Smith pushes for Lyme disease bill as NJ cases skyro... mark_denton2006@yahoo.com lyme 0 Tue Jul 18, 2006 7:39 pm
No new posts Gum disease can lead to more than unhealthy mouth Roman Bystrianyk dentistry 1 Tue Jul 18, 2006 4:27 pm

Copyright © 2004-2005 DeniX Solutions SRL
Other DeniX Solutions sites: email marketing campaigns , electronics forum, Science forum, Unix/Linux blog, Unix/Linux documentation, Unix/Linux forums


Powered by phpBB © 2001, 2005 phpBB Group
[ Time: 0.0139s ][ Queries: 16 (0.0040s) ][ GZIP on - Debug on ]